Proteomics
What do we do?
Proteomics and metabolomics are the systematic study of the unique protein and metabolite fingerprints of biological systems. The TMRC proteomics and metabolomics Laboratory uses “state of the art” tandem mass spectrometry to obtain quantitative protein and metabolic profiles from clinical samples (as well as samples from animal models) to develop new approaches to early diagnosis, prognosis, patient selection, therapeutic monitoring and target identification for diseases. This laboratory also works closely with the TMRC genomics and immunoassay laboratories to gain wider systematic view in diseases.
Facilities
The laboratory is situated in the level 7 of TMRC Building at Ninewells Hospital Campus ( link to map). The laboratory is equipped with
- LC systems including a nano-LC and a micro/nano- switchable LC)
- Mass spectrometers including a Thermo LTQ-Orbitrap and a TSQ Quantum Ultra
The laboratory specializes in semi-quantitative protein/metabolite profiling and absolute protein/metabolite quantification (using stable isotopic standard as internal controls). The team has implemented rigorous procedure to ensure experimental conditions were well-controlled from samples to data output.
Meet the Team
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Jeffrey Huang, PhD
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Osama Albarbarawi, PhD
Osama completed his PhD degree in 2002 from University of Granada (Spain) developing single-use sensors for the determination of antibiotics (Quinolones). Followed by a year lecturing in Hebron University; and then a post-doctoral position using LC-MS/MS and GC-MS/MS techniques for the analysis of pesticides in fruits and water. Before joining TMRC in April 2007, Osama Post-doctoral position at medical school -Toxicology Unit- Newcastle University Involved the development of a sensitive biomarker for human organophosphates exposure by monitoring the percentage of Acetylcholinesterase enzyme inhibition using MALDI-TOF/TOF technique, with specific application to the treatment of Alzheimer’s disease. |
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Abdel Atrih, PhD
Abdel Atrih is the Post doctoral scientist within the Proteomics group having joined the TMRC in April 2007. Following his Baccalaureate in Life Sciences in Algeria, Abdel attained his MSc and Ph.D in the INPL, Nancy, France in 1990 and 1994 respectively. Between 1994 and 2001 Abdel studied and published extensively on bacterial endospore peptidoglycan structures. Between 2001 and 2005 Abdel joined the University of Dundee where his research focused on the characterisation of glycan structures of Trypanosoma Brucei flagellar pockets. Prior to joining the TMRC Abdel studied the structure – function relationships of animal proteoglycans including Heparan sulphate at the University of Liverpool. |
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Alun Barton, BSc
Alun Barton is a scientific officer within the TMRC Proteomics group. Alun completed a sandwich degree course in Biomedical Technology at Sheffield Hallam University in 1991 and also gained a postgraduate diploma in Biotechnology from Abertay University (Dundee) in 1993. Alun enjoyed research in a number of labs within Dundee University and Ninewells hospital prior to joining CXR Biosciences in 2002, where he gained experience in radiolabelled metabolism assays, LC/MS qualitative assays and LC/MS/MS small molecule quantification in whole blood and brain tissue. |
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Fiona Wilson, PhD
Fiona is a post-doctoral scientist working on a MRC/Wyeth funded project in collaboration with Dr Calum Sutherland and Professor Mike Ferguson in University of Dundee. Fiona’s major research interest is molecular signaling in metabolism. She gained her PhD in University of Aberdeen and spent three years in Baylor college of Medicine as a post-doctoral scientist. She has published six peer reviewed papers in the field and won a “Postdoctoral Fellow Research Award” from American Society for Nutrition. |
